Localization and characterization of somatostatin binding sites in the mouse retina.

Localization of Epidermal-Type Fatty Acid Binding Protein (E-FABP) in Degeneration and Regeneration of Sciatic Nerve after Crush Injury in Mouse

Purpose:The regeneration of axon and myelin sheet after crush injury of peripheral nerves involves interaction of several types of cells, including Schwann cells, monocyte, macrophage and fibroblast. Among them, haematogenous macrophages invading into the peripheral nervous systein play a major role in myelin uptake during Wallerian degeneration. Materials and Methods: In this study 35 C57/BL6 ...

The Characterization and Regulation of Benzodiazepine Binding Sites in the Mammalian Retina, Cerebral Cortex and Kidney

The binding of [JH]flunitrazepam (FLU) to membranes prepared from mammalian brain, retina and kidney was investigated by means of conventional filtration assay techniques. In the mouse brain a study of the ontogeny of [ H]FLU binding was conducted. Specific [ H]FLU binding was present early in development and there was a rapid increase in receptor density (Bmax) during the first 2 weeks of neon...

Identification and characterization of somatostatin receptors in neonatal rat long bones.

Somatostatin (somatotropin release inhibiting factor; SRIF) has widespread functions as a modulator of neural activity as well as of endocrine and exocrine secretion. In the present paper, the binding characteristics of somatostatin receptors have been investigated in rat long bones using the stable analogue, 125I-SDZ 204-090, as a ligand. Binding studies revealed the presence of a single class...

Localization and Pharmacological Characterization of Somatostatin Recognition Sites in the Brain

Cortistatin radioligand binding in wild-type and somatostatin receptor-deficient mouse brain.

Cortistatin-14 (CST-14) is a recently discovered member of the somatostatin family of neuropeptides. It shares 11 of its 14 amino acids with somatostatin-14 (SRIF-14). In the present study, binding sites for cortistatin-14 in the mouse brain were examined and compared to those for somatostatin using iodinated cortistatin-14 and iodinated somatostatin-14. By in vitro receptor autoradiography, hi...